| 시설장비 설명 |
저진공표준기인 초음파간섭 수은주압력계외 비교되는 표준기
A method for carbonic anhydrase II (CA II) absolute quantification in human serum is presented. This method is based on high-performance liquid chromatography (HPLC)-Chip microfluidic device incorporating a nanoelectrospray source interfaced to a triple quadrupole mass spectrometer. The fraction containing CA II was isolated by preparative reversed-phase HPLC and peptides obtained from the tryptic digest of the protein mixture were separated by the HPLC-Chip system. The multiple-reaction monitoring acquisition mode of a selected suitable CA II peptide and peptide internal standard allowed the selective and sensitive determination of a CA II. Absolute recovery of the method was 52±12% while analytical recovery was 81±10%. For the eight samples analyzed the matrix effect was found to be only 14±6%. A comparison among three regression lines type which were obtained by external calibration matrix-matched calibration and standard addition method respectively demonstrated that the fir |
